INTRAGENOTYPIC CHARACTERIZATION OF SEVEN ACCESSIONS OF TARWI (Lupinus mutabilis Sweet.) USING ISSR MOLECULAR MARKERS

Abstract

The objective of this research was to characterize seven accessions of Tarwi (Lupinus mutabilis Sweet), from the INIA germplasm bank. Molecular markers of simple sequence inter-repeats (ISSR) were used, selecting 10 primers to amplify the DNA of 91 individuals (seeds) under study, 13 per accession, detecting a total of 247 loci. These primers were highly informative with an average polymorphic information content (PIC) of 0.33. The main factor of in situ gene flow, among these accessions, was the exchange or sale of seeds at fairs or markets close to the collection area, due to the ruggedness of these places. The predominant autogamous pollination of L. mutabilis and the grouping of the accessions in the phenogram based on the similarity analysis, showed the high probability of the increase in outcrossing during crop regeneration, being considered one of the main factors of gene flow during this period.